University of Manitoba Research Study

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Egg Antibodies in prevention of diarrhea

Improvement of Growth performance in piglets after Feeding Nursery diet supplemented with HYPER-EGG K88® and infecting piglets with ETEC K-88 bacteria

Study performed: University of Minnesota
Number of piglets used in the study: Thirty 18-day old piglets and six were in each group.
Feeding regimen: Each group were fed nursery diet supplemented with EG, (4Kg control egg powder from unimmunized group/T of feed), or ABEG 0.2%, (2Kg HYPER-EGG K88®/T of feed), or ABEG 0.4% (4Kg HYPER-EGG K88®/T of feed), or ABDEX 0.2% (1Kg of HYPER-EGG K88® + 1Kg Dextrose/T of feed), or ABDEX 0.4% (2Kg of HYPER-EGG K88®+ 2Kg Dextrose/T of feed), for 14 days, from day 1 to day 14, post-weaning. Piglets were fed normal diet during day 14-28.
Infection with ETEC K88: All piglets were infected orally on day 7 post-feeding with 1012 CFU of ETEC K88. • Study parameters: Clinical response (incidence of diarrhea, severity of diarrhea, enumeration of ETEC K88 from rectal swab) and changes in J. H. Hare & Associates Ltd.
1-270 Roslyn Road. Winnipeg, Manitoba, R3L 0H3 CANADA. Telephone 1-800-661-4273 Fax (204) 477-8301
E-mail: john@hyper-egg.com

Improvement of Growth Performance of post-wean piglets following feeding with nursery diets supplemented with HYPER-EGG® K-88

Study performed at the Department of Animal Sciences, University of Manitoba, Winnipeg, Canada. -September-December 2010.
Study Directors: Dr. Martin Nyachoti, Professor, Department of Animal Sciences, University of Manitoba and Dr. Pradip K. Maiti, Vice-President & Chief Scientific Officer, J.H. Hare & Associates Ltd.
Study Personnel: Dr. Kiarie Elijah, University of Manitoba

Study Objectives: To determine the effect of supplementing pig starter diets with HYPER-EGG® K-88 product on growth performance of newly-weaned piglets
Methods: Three groups of 21-day old piglets, 24 piglets in each group, were used in the study. Piglets in Group 1 were fed with Phase I nursery diet; piglets in Group 2 were fed with Phase I nursery diet supplemented with 0.3% HYPER-EGG K-88, when piglets in Group 3 were fed with diet supplemented with 5% Spray-dried plasma, for 14 days, day 0- day14. Piglets in all three groups were then fed with phase II diet without any supplement, as illustrated in Table -1. The growth performance and feed intake were monitored on days, 7, 14 and 25, post-feeding, to assess growth performance of piglets.

Results: The growth performance of piglets fed with both HYPER-EGG and Plasma showed improvement on day 7, 14 and continued on day 25, as compared to the control piglets. The average body weight was increased by 1.1Kg on day 25 in the HYPER-EGG fed animals over the control group. Interestingly, the highest ADG (428g) was achieved on day 25, in the group that was fed with HYPER-EGG K-88 for 14 days. The results are shown in Tables 2-3 and Figures 1-2.
Conclusions: The growth performance of post-wean piglets have been improved by 1.1 kg on day 25, after feeding piglets for 14 days with nursery diet supplementing with 3Kg of HYPER-EGG K-88® per metric ton of feed.

Table 1: Diets composition, as is

Ingredient, % Phase I/Control Phase I/HYPER-EGG® K-88 PhaseI/Spray dried plasma Phase II/Control
Soybean meal 17 17 17 24
Canola meal - - - 6
Wheat 10.71 10.71 10.71 14.49
Corn 38.50 38.50 38.50 44.20
Hyper EGG® - 0.30 - -
Spray dried plasma - - 5 -
Dried whey 16.21 16.10 12.29 5.00
Menhaden Fish meal 9.00 9.00 4.00 0.00
Lactose 2.00 2.00 4.50 0.00
Limestone 0.25 0.26 0.60 1.00
Monocalcium phosphate 0.40 0.40 0.95 1.00
Vegetable oil 3.30 3.10 4.00 2.50
Iodized salt 0.30 0.30 0.30 0.30
Vitamin-mineral premix 1 1 1 1
BioLysine 0.85 0.85 0.65 0.36
DL-Methionine 0.20 0.20 0.22 0.05
L-Threonine 0.28 0.28 0.28 0.10
ME, Kcal/kg 3,364 3,361 3,360 3,268
SID CP, % 20.64 20.63 20.85 19.25
SID Lys, % 1.36 1.36 1.36 1.01
SID Met, % 0.54 0.54 0.50 0.32
SID Met + Cys, % 0.80 0.80 0.85 0.62
SID Thr, % 0.97 0.98 1.04 0.71
SID Trp, % 0.21 0.21 0.24 0.21


Table 2: Effect of HYPER-EGG® K-88 product on growth performance of weaned pigs - day 0-14

Control HYPER-EGG® K-88 SD-Plasma SEM P-value
Body weight, kg
Initial Day 0 5.86 5.85 5.83 0.213 0.994
Day 7 6.33 6.42 6.60 0.251 0.747
Day 14 7.35 7.66 8.05 0.294 0.272
Day 0-7
ADG, g/d 76.5 94.6 127.2 14.56 0.074
ADFI, g/d 121.0 160.6 152.3 15.95 0.213
G:F, g/g 0.716 0.643 0.824 0.135 0.645
Day 7-14
ADG, g/d 171.4 207.50 242.90 22.63 0.116
ADFI, g/d 260.5 276.9 276.9 22.30 0.627
G:F, g/g 0.673 0.750 0.869 0.089 0.317
Day 0-14
ADG, g/d 124.0b 151.0ab 185.1a 11.95 0.009
ADFI, g/d 190.8 218.8 221.9 12.88 0.204
G:F, g/g 0.695 0.696 0.847 0.057 0.132

n=4 pigs/pen
ab Within a row means with different letter signs significantly different, P less then 0.05


Table 3: Improvement of growth performance of nursery pigs on day 25 after feeding with HYPER-EGG K-88 product for 14 days during day 0-14

Control HYPER-EGG® K-88 SD-Plasma SEM P-value
Body weight, kg
Initial day 14* 7.36 7.59 8.06 0.326 0.332
Final day 25 10.07 11.8 12.0 0.527 0.176
ADG, g/d 345 428 382 28.7 0.094
ADFI, g/d 535b 660a 587ab 41.7 0.044
G:F, g/g 0.650 0.654 0.652 0.06 0.830

* used as covariate in statistical analysis. n=3pigs/pen. ab Within a row means with different letter signs significantly different, P less then 0.05